The pyridine nucleotide metabolism of mitochondria incubated with and without added substrates and metabolic inhibitors.

The experiments described previously (Birt & Bartley, 1960a, b) have shown that the amounts and state of reduction of the endogenous pyridine nucleotides of rat-liver mitochondria are controlled by the components of the incubating medium. This paper is concerned with a general study of the effects of the temperature of incubation, the addition of various substrates, 2:4-dinitrophenol and amytal on the behaviour of the pyridine nucleotides in these particles; in particular, factors altering the dynamic steady-state levels of reduced nucleotide (by changing rates of oxidation or reduction of the coenzymes, or both) have been investigated.